This pipeline is designed to process bisulfite sequencing data. It is compatible with various library preparation methods such as targeted methylation sequencing, RRBS, or WGBS. It can help the user evaluate the quality of their samples or libraries, quantify reads, find differentially-methylated sites and regions, and more. Please find the sample report here
This pipeline is originally adapted from published BiSulfite Bolt pipeline v1.5.0. Zymo Research and Pacific Informatics made significant contributions in the adaption effort. This mainly include making a Nextflow pipeline running the BSBolt software, and the improvement of the report and its documentation.
This pipeline is built using Nextflow. A brief summary of pipeline:
FastQC)Cutadapt)BiSulfite Bolt)BiSulfite Bolt)